Publications

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Kanama, S., Viljoen, A.M.., Kamatou, G.P.P., Chen, W., Sandasi, M., Reza Adhami, H., Van Wyk, B-E. 2015. Simultaneous quantification of anthrones and chromones in Aloe ferox (“Cape aloes”) using UHPLC–MS. Phytochemistry Letters 13: 85-90.

Aloe ferox is an important medicinal plant indigenous to South Africa where it has primarily been used as a purgative and also to treat skin disorders. Modern day uses include; health (tonic) drinks, flavourant in alcoholic beverages, treatment of digestive disorders and inclusion in cosmetic formulations. Due to increased commercialization of aloe products, a rapid, sensitive and reliable quality control method for the raw material has become a necessity. Ultra-high performance liquid chromatography coupled to mass spectrometry (UHPLC–MS) was investigated as an ultrafast, accurate, and sensitive method in the quantification of chromones (aloeresin A, aloesin) and anthrones (aloin A and B) in 101 A. ferox exudates. The method was validated for accuracy, precision and limits of detection and quantification. The calibration curves showed good linearity (R2>0.99) within the concentration range and the recoveries of the four analytes ranged from 100–114% with the relative standard deviation lower than 2%. The limits of detection and quantification for all compounds were within a range of 0.21.1 µg/ml. Quantitative determination of the four biomarkers showed high levels of aloeresin A (129.0–371.6 µg/mg) and aloesin (111.8–561.8 µg/mg) while aloin A (21.3–133.4 µg/mg) and aloin B (18.4–149.7 µg/mg) were present in lower amounts. Hierarchical cluster analysis revealed two major groups within the dataset. However geographical locality was not a major factor in the clustering observed. This validated, ultra-fast method for simultaneous quantification of chromones and anthrones present in A. ferox is recommended for routine quality control analysis.



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