The methods currently used to authenticate and validate H. gordonii raw material are laborious and require skilled personnel. The development of a rapid and simple authentication and validation method for raw materials is of utmost importance to curb or eliminate the high degree of adulteration of H. gordonii products. For any compound to exert a pharmaceutical effect, it needs to be absorbed into the systemic circulation in order to become available at the site of action. Since H. gordonii products are administered in different dosage forms via different routes of administration, it is necessary to determine if P57 is transported across the intestinal and buccal mucosa.
The aims of the research project are to develop and optimise a rapid quality control method for H. gordonii raw material and products and to determine whether components of H. gordonii (including P57) are absorbed through the intestinal and buccal mucosa.
• To develop a chemical fingerprint for H. gordonii using high performance thin layer chromatography (HPTLC).
• To confirm the presence of and quantify the amount of P57 (active) in various samples using liquid chromatography coupled to mass spectrometry (LC-MS).
• To develop a calibration model using the quantification data in conjunction with the near infrared (NIR) and fourier transform mid infrared (FT-MIR) spectroscopy data.
• To develop a method to detect adulteration of H. gordonii raw material and products (HPTLC, NIR and FT-IR) and to confirm the presence or absence of P57 (HPTLC) and to quantify P57 (NIR and FT-MIR).
• To determine whether components of H. gordonii (including P57) are transported across the intestinal epithelium by using the Caco-2 cell culture model, and across pig intestinal mucosa in Sweetana-Grass diffusion chambers.
• To determine whether components of H. gordonii (including P57) are transported through pig buccal mucosa in Sweetana-Grass diffusion chambers.
Principal supervisor: Prof AM Viljoen
Co-supervisor: Prof JH Hamman